Yes, you can always run in "manual" mode....editing the protocol to "titration" rather than "antibody". That does indeed allow you to manually apply the antibody when the instrument signals its time to do so.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Milne, Katy Sent: Tuesday, October 14, 2008 12:45 PM To: [EMAIL PROTECTED]; [email protected] Subject: RE: [Histonet] Expiration dates Hi Amy, I use the Ventana Discovery and I add my antibodies manually as I'm in research and don't feel like shelling out extra for empty containers to put my Abs in. Do you have an option on the Benchmark to switch the primary Ab step to "titration" or something like that (can't remember what the exact step name is but not the autodispense one). I don't even know if that's an option on the Benchmark but with the Discovery, I can just open that machine and add it straight at that step. Perhaps you could get your antibody out of the dispensor and try it that way. Katy Milne Deeley Research Centre - Message: 4 Date: Mon, 13 Oct 2008 07:04:10 -0400 From: "Senn, Amy R" <[EMAIL PROTECTED]> Subject: [Histonet] Expiration dates To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
