We give our Hematology department Superfrost+ slides to use for smears, because we had the same problem. We also went back to the old fashioned way of fixing the smears. We use a coplin jar with a small piece of papertowel in the bottom and place a few drops of formalin in the coplin jar. The smears are fixed in formalin fumes. We had a lot of smears that were not staining for iron before using the methanol method.
Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Georgine Whitman Sent: Friday, November 14, 2008 9:51 AM To: [EMAIL PROTECTED] Subject: [Histonet] Bone Marrow smears fixation we are trying to do an iron stain on our bone marrow smears on the Ventana Nexus. We are having trouble with the specimen staying on the slide. I personally have not done iron on smears in years. Our hematology department has been doing them by hand and they are fixed in methanol alcohol. Please Help Thank You Georgine _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
