Michele, You could try 0.5% Oil Red O in 60% triethylphosphate. That works really well here. I've also used a formulation I found in Humanson, the same amount of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is much cleaner than the ethanol. (There was a precipitate on the slide.) I can't avouch for isopropyl as I used ethanol, but it is good to know there's an alternative in case we run out of triethylphosphate.
Amos Brooks Message: 10 Date: Mon, 17 Nov 2008 11:56:45 -0600 From: "Michele Wich" <[EMAIL PROTECTED]> Subject: [Histonet] Oil Red O despair To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
