Hello everyone! I was wondering if anyone has ever pre-incubated the antibody with normal Mouse IgG to get rid of the unspecific staining. The antibody I use crosreacts with mouse , and both human and mouse tissues stain within a xenograft. I've heard that one can get rid of the mouse staining by incubating with murine IgG. Has anyone ever done that and if so, you mind sharing some info? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
