Tom: Dehydrate with graded isopropyl alcohol and clear with mixtures 5:1 and 2:1 of isopropyl and mineral oil (at 50ºC), followed by pure mineral oil (50ºC) and your regular infiltration with paraffin. You will eliminate xylene totally and the used mineral oil will be disposed off mixed with the used paraffin as a solid. If you want I can send you further details and a protocol. René J.
--- On Thu, 1/22/09, Tom McNemar <[email protected]> wrote: From: Tom McNemar <[email protected]> Subject: [Histonet] Xylene substitute To: [email protected] Date: Thursday, January 22, 2009, 6:04 AM Wondering what folks are using as a xylene substitute. We have used Americlear for many years and it has worked well. I would like to find something that is biodegradable and doesn't have such a strong odor. Any suggestions or recommendations? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 [email protected] www.LMHealth.org _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
