We also run a p63/HMCK/p504s dual stain cocktail on prostate cores. Easy to
set up and results look great.
THe Kappa/Lambda dual stain works, too. I find the red chromagen (lambda)
in this setting is a little less precise, tho'.
REgards
Piero Nelva
Anatomical PAthology
Monash Medical Centre
----- Original Message -----
From: "Gudrun Lang" <[email protected]>
To: <[email protected]>
Cc: <[email protected]>
Sent: Friday, January 23, 2009 3:56 AM
Subject: AW: [Histonet] double staining on the ventana XT
Hi Jennifer,
we perform the doublestain routinly. The preferred protocol is CK HMW/p504s
on prostata biopsies.
The protocol is easy to establish and the staining results are very good.
The run lasts about 4 hours and we do it overnight. You need the Red and the
DAB ultraview kit. No extra reagens.
Gudrun
-----Ursprüngliche Nachricht-----
Von: [email protected]
[mailto:[email protected]] Im Auftrag von
[email protected]
Gesendet: Donnerstag, 22. Jänner 2009 01:41
An: [email protected]
Betreff: [Histonet] double staining on the ventana XT
Greetings,
We are looking into doing some immuno double staining. I searched the
archives and found a few similar inquiries but no responses. Anyone with
experiences to share I would love to hear what you have to say. Everything
from what not to do to what works well for you.
Many Thanks,
Jennifer
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