Look at the mountant you are using. Some are not as colour-fast as one would expect.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of shazana hilda Sent: Wednesday, 4 February 2009 2:23 PM To: McMahon, Loralee A; histonet forum Subject: Re: [Histonet] query regarding IHC slide Dear Loralee, Unfortunately I'm using DAB and yet still faded. This is the first time I'm facing this problem. Thank You. Shazana Hilda ShamsuddinUniversiti Sains Malaysia Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: [email protected] ________________________________ From: "McMahon, Loralee A" <[email protected]> To: shazana hilda <[email protected]> Sent: Tuesday, February 3, 2009 11:47:06 PM Subject: RE: [Histonet] query regarding IHC slide [Histonet] query regarding IHC slide Are you using DAB or AEC. AEC fades and DAB does not. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: [email protected] on behalf of shazana hilda Sent: Mon 2/2/2009 9:10 PM To: [email protected] Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciated. Regards. Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
