Suzanne: I no longer use Sanderson's, however we did have nice results on plastic embedded sections, in conjuction with other calcified stains. Use caution in over-staining. I do not recall ever reading about usage on paraffin. The Villanueva Osteochrome bone stain can also be used on calcified - we used it with fluoro labels, and then viewed using confocal microscopy with beautiful architecture and active new bone formation. Vicki K _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
