I agree with Tony that a -70 freezer is ideal. We wrap our samples in aluminum foil and then place in a plastic container. If you only have access to a -20 freezer, it's helpful to place cold packs in the freezer, near where you will store the muscle samples. When a -20 freezer cycles, the temperature can fluctuate enough to create artifact. We've found that if we keep several disposable cold packs in a -20 that we can keep the temperature more consistent. Good Luck!
Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Tony Henwood [mailto:[email protected]] Sent: Tuesday, March 03, 2009 3:59 PM To: [email protected]; [email protected] Subject: RE: [Histonet] Storage of muscles Minus 70 degrees A normal minus 20 freezer tends to dessicate the tissue Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Adrienne Anderson Sent: Wednesday, 4 March 2009 7:28 AM To: [email protected] Subject: [Histonet] Storage of muscles Hello, We are having problems with artifact after our muscles have been stored for long periods of time. Can anyone tell me how they store their muscle specimens for long-term? Thank you, Adrienne Anderson Duke University Health System [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
