Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. René J.
--- On Wed, 3/4/09, Va Paula Sicurello <[email protected]> wrote: From: Va Paula Sicurello <[email protected]> Subject: Re: [Histonet] problem eyes To: "[email protected]" <[email protected]>, "MargaretPerry" <[email protected]>, [email protected] Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa <[email protected]> wrote: > From: Rene J Buesa <[email protected]> > Subject: Re: [Histonet] problem eyes > To: "[email protected]" <[email protected]>, "Perry, Margaret" <[email protected]> > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > rené J. > > --- On Wed, 3/4/09, Perry, Margaret <[email protected]> > wrote: > > From: Perry, Margaret <[email protected]> > Subject: [Histonet] problem eyes > To: "[email protected]" > <[email protected]> > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
