Hi Vanessa,
I have read in at least one online protocol and have also been advised by
our excellent and experienced (former) histotech who used to be here at UB
NOT to warm the slides without air-drying first. We typically air-dry the
slides, yes, standing up, overnight, before warming them.
Merced
--On Wednesday, March 04, 2009 4:35 PM -0500 "Vanessa J. Phelan"
<[email protected]> wrote:
Hi everyone,
Quick question, I am finding after cutting a lot of slides for H&Es onto
VWR Superfrost Plus slides (these are the only slides we have at the
moment) that when I lift sections from the water bath ( of distilled
water) a water bubble stays under the sections on the slide. When I lie
them flat on the hot plate to dry the water bubble is tending to distort
the tissue by escaping out through he tissue, eventually. I have not
experienced this before, the water has ran off the slide no problem.
Would it be the slides that are the problem or anything I might need to
add to the water?
I have just ordered a histology oven, this may be the solution...drying
the slides standing up!
Vanessa
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
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