Re: Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think Tuj1 is restricted to immature neurons inside the brain ! though it stains well with retinal ganglion cell....
I am also seeking for a nice marker for axon ...both normal and regenerating...GAP-43 is one choice for regenerating axons...but a bit focused on the growth cone, and the staining is not strong on transporting proteins - due to the tiny amount? I am not sure how NF-200 or NF-96/160 work.... ANyone can suggest better markers for axon IHC staining? both normal and regenerating. _______________________________________________________________________________________________ Axons stain nicely with beta 3 tubuline (TUJ). We've used the following antibodies Monoclonal anti-beta-tubulin isotype III from Sigma 1/1000 DAKO envision (révélation PAL or HRP) (ref : T8660) or Polyclonal (rabbit) antibody against neuronal class III beta-tubulin from covance research commercialise par BABCO Berkeley antibody company au 1/1000 DAKO envision (révélation PAL or HRP) (ref : PRB-435P) We used paraffin slices of PFA fixed rat embryos and adult rat brain, microwaving in citrate buffer. These antibodies also work nicely with IF Good luck! Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Labége Innopole 31676 LABEGE CEDEX FRANCE [email protected] tel : (33)561004179 fax :(33)561004001 2009-03-05 TF
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