If PIER is needed, we use Proteinase K, but we avoid it, if possible. Citrate Buffer is historically the next buffer, that got generally used. Few years later with investigations about Ca-Ions involved in fixation, the EDTA-buffers with high pH appeared. It was shown, that most of the diagnostic antibodies work well with high pH retrieval. So my first choice is the EDTA-buffer when introducing a new antibody. Although the most datasheets require Citrate buffer. Gudrun
-----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Margaryan, Naira Gesendet: Freitag, 27. März 2009 20:38 An: [email protected] Betreff: [Histonet] Trypsin versus other AR-s Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
