I am just starting to bring IHC in house to our new lab, I have been working with the tech staff at Cell Marque. They sent me a "cuisinart" pressure cooker to work with, it is pretty nice.. has a digital timer.. gets the job done. I have only been working up H.pylori at this time, with a pre-dilute antibody.. but I have used the pressure cooker at high temp, 15 mins with success. It allows you to pick low/high also.. Much cheaper than other models I looked at.
Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 [email protected] The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Jennifer Campbell Sent: Monday, March 30, 2009 9:44 AM To: [email protected] Subject: [Histonet] pressure cookers for IHC Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type of pressure cooker to use? Protocol used as far as the temp and for how much time? Any info would be appreciated. Thanks, Jen C. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
