I don't think the bone matrix will re-calcify with normal bone histo solutions. What exactly are you using to decalcify the tissue? Hardening can occur at many steps following decal. I stopped using a commercial decalcifying agent (which consisted of formic acid with chelating agents added) because my tissue samples would harden more rapidly after dehydration, clearing and infiltration (paraffin) and wouldn't infiltrate properly. Not sure if it was the chelating additives or not, but I went back to using Formic A solution with 5-10% formic depending on the type of tissue.
Matt _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
