Dear Histonetters, I was wondering whether anyone had success in Fli-1 immunohistochemistry on formalin-fixed, paraffin embedded human sections.
I tried two polyclonal, commercial antibodies from abcam (ab15289) and Santa Cruz Biotech's Fli-1 (C-19, sc-356) without any positive stainings. Both antibodies were recommended for application on paraffin sections. Abcam's application notes even mentioned that perform enzymatic antigen retrieval BEFORE commencing with HIER procedure. The procedure still did not give any stains and the morphology was unacceptable. I tried with/without HIER with different buffers as well. I used primary dilutions 1:50 - 1:200 as recommended and I am using Leica's BondMax Autostainer. Any comments would be appreciated. Young Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
