Hi. I use DPX from VWR for all of my std dye-based stainings (such as H&E, Alcian blue/PAS) and also for my DAB IHC: 500ml for 35UK£. I note that the equivilent Zymed product, Histomount is a similar UK price.....but, for 15ml. Perhaps I am wrong? I have used Histomount and DPX and do not notice any difference to the stainings under the microscope. carl
________________________________________ From: [email protected] [[email protected]] On Behalf Of [email protected] [[email protected]] Sent: 30 April 2009 18:03 To: [email protected] Subject: Histonet Digest, Vol 65, Issue 51 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Colorado Society of Histotechnology - Meeting May 8th & 9th in Colorado Springs (McGinley,John) 2. NSH Call for Awards Applications/Nominations (Mitchell Jean A) 3. Rolling sections (Pixley, Sarah (pixleysk)) 4. mounting media for IHC (Rashmil) 5. MMR protein antibodies for Lynch Syndrome (Taylor, Jean) 6. Reusing Citrate Antigen Retrieval Buffer (Jason McGough) 7. Position In Florida (Alyssa Peterson) 8. Re: Reusing Citrate Antigen Retrieval Buffer (TF) 9. Re: mounting media for IHC ( TF ) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Apr 2009 13:20:27 -0600 From: "McGinley,John" <[email protected]> Subject: [Histonet] Colorado Society of Histotechnology - Meeting May 8th & 9th in Colorado Springs To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi, The Colorado Society of Histotechnology (CSH) meeting will be held at the Cheyenne Mountain Resort in Colorado Springs, May 8th and 9th, 2009. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2009/meeting.htm. Online registration is available and payment can made on the day of the meeting (check only. Sorry, no credit cards accepted at this time). Thank you and I look forward to seeing you at the meeting. Regards, John McGinley CSH Secretary ------------------------------ Message: 2 Date: Wed, 29 Apr 2009 23:17:52 -0500 From: "Mitchell Jean A" <[email protected]> Subject: [Histonet] NSH Call for Awards Applications/Nominations To: <[email protected]> Message-ID: <2108aecb05dbff48a9c436a792155740010c3...@uwhc-mail03.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Last Call for Nominations/Applications: You still have time to make the NSH 2009 Awards/Scholarship May 1st deadline! Each year, NSH recognizes the commitment and achievements of individuals in the histology profession, spotlights students and their achievements, and applauds the innovation of members dedicated to advancing our science. We invite you to apply and or nominate a professional for the 2009 NSH Awards and Scholarship Program. Please review the detailed descriptions of each Award and their respective criteria at www.nsh.org and then nominate the deserving professional. Be sure to submit applications by FRIDAY; the nomination deadline of May 1, 2009. Jean Mitchell - Chair, 2009 NSH Awards Committee ------------------------------ Message: 3 Date: Thu, 30 Apr 2009 09:48:05 -0400 From: "Pixley, Sarah (pixleysk)" <[email protected]> Subject: [Histonet] Rolling sections To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" At one time, when we were using knives (not disposable blades), and we were using a roller plate, I found that the Shandon (now Thermo Scientific) M1 Embedding Matrix: http://www.thermo.com/com/cda/product/detail/1,1055,21902,00.html appeared to cause less rolling than OCT. You could try that. (However, now my lab is now using OCT, with disposable blades, without a roller plate, and they are not having difficulties cutting rat brains.) Another thing to check is to make sure all the settings are correct, as in previous responses: temperature, thickness, etc. Also, be sure that the knife angle is adjusted perfectly for your tissue and your temperature. Subtle changes in angle can help sectioning in many ways. Finally, if static electricity is a problem, you can try removing gloves (if you are not trying to be RNase free). Gloves can sometimes, depending on the type, increase static. Best, Sarah ------------------------------ Message: 4 Date: Thu, 30 Apr 2009 07:40:34 -0700 (PDT) From: Rashmil <[email protected]> Subject: [Histonet] mounting media for IHC To: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=iso-8859-1 Hi Is there a mounting solution that is better than another for IHC slides? I am using Histomount from ZYMED. And have no problems with it. But I need to order some more and was wondering if there was one product that was better than the other? ?I was especially interested to hear about Ecomount from Biocare. Thanks, Rashmil ------------------------------ Message: 5 Date: Thu, 30 Apr 2009 09:47:42 -0500 From: "Taylor, Jean" <[email protected]> Subject: [Histonet] MMR protein antibodies for Lynch Syndrome To: "'[email protected]'" <[email protected]>, "'[email protected]'" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi Everyone, I'm looking for information about MLH1, MSH2, MSH6 and PMS2 and any other IVD antibody that labs are using to identify Lynch Syndrome. Would appreciate any information regarding antibody source, retrieval methods, dilutions, etc... Thank you! Jean Taylor, HT(ASCP) QIHC IHC Tech Meriter Health Services 36 S. Brooks St. Madison, WI 53715 ------------------------------ Message: 6 Date: Thu, 30 Apr 2009 09:09:10 -0600 From: "Jason McGough" <[email protected]> Subject: [Histonet] Reusing Citrate Antigen Retrieval Buffer To: <[email protected]>, <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) [email protected] ------------------------------ Message: 7 Date: Thu, 30 Apr 2009 11:26:13 -0400 From: Alyssa Peterson <[email protected]> Subject: [Histonet] Position In Florida To: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=ISO-8859-1 Hello, My name is Alyssa Peterson, and I am the director of lab/pathology recruitment at Allied Search Partners, and I wanted to follow up with you in reference to a full time/permanent job opportunity for a talented histotechnologist. The position is in* Auburndale, Fl area. **If this is not the job for you, however, you are interested in exploring new opportunities please send me a copy of your updated resume and what you are looking for*. Also, we offer to pay you up to $1000 for any referral that we place in a position, so please forward this to anyone who seems fit for this position and have them contact me right away! *Position: Histotechnologist* * * *Shift* *Full time, day shift* * * *Job Description:* Responsible for performance of specimen processing, embedding, cutting, routine and special histologic staining, frozen sectioning and mounting of preparations from surgical and autopsy materials for microscopic interpretation by the Pathologist. In addition, measures and describes needle core biopsies of the breast, liver, and all endoscopy biopsies. Assists Pathologist by measuring, apportioning muscle biopsies; sural nerve biopsies, and needle core biopsies of the kidney. Implements and tests new techniques and procedures. The Histotechnologist identifies tissue structures, cell components, and their staining characteristics, relating them to physiological functions, making judgments concerning the results of quality control measures. Also prepares fresh unfixed tissue for transporting to a reference lab. Requirements & Working Conditions ? High School Diploma Required. ? Completion of structured training and education in Histology. ? Licensed by the State of Florida as a Histology Technologist. ASCP certification preferred. ? Ability to stand and walk for approximately twenty percent of work time when preparing tissues, and ability to lift, carry and manipulate solvents/solvent containers, supplies, or equipment to a maximum of 25 lbs. ? Is on-call occasionally or adjusts work schedule to respond for frozen sections. ? Works in a laboratory histology section with frequent (up to thirty percent of work time) exposure to unpleasant odors, chemicals and bio-hazardous specimens. ? Potential exposure to cuts on hands and fingers from microtome knives but hazards are limited when established handling procedures are followed. -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ------------------------------ Message: 8 Date: Fri, 1 May 2009 00:24:12 +0800 From: "TF" <[email protected]> Subject: Re: [Histonet] Reusing Citrate Antigen Retrieval Buffer To: "Jason McGough" <[email protected]>, "[email protected]" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="gb2312" We never tired that but I think you should not do that. For the sake of the 2nd batch of sections; also, the solution pH, concentration, ion strength after boiling should have changed/ 2009-05-01 TF ???????? Jason McGough ?????????? 2009-04-30 23:15:31 ???????? [email protected]; [email protected] ?????? ?????? [Histonet] Reusing Citrate Antigen Retrieval Buffer We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 1 May 2009 00:25:10 +0800 From: " TF " <[email protected]> Subject: Re: [Histonet] mounting media for IHC To: " rashmil_histotechnology " <[email protected]>, " histonet " <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="utf-8" Hi, we are using DAKO fluorescent mounting medium. We also have DAPI-mouting medium so that you dont have to stain for DAPI/hoechest. 2009-05-01 TF ???????????? Rashmil ??????????????? 2009-04-30 22:44:48 ???????????? histonet ????????? ????????? [Histonet] mounting media for IHC Hi Is there a mounting solution that is better than another for IHC slides? I am using Histomount from ZYMED. And have no problems with it. But I need to order some more and was wondering if there was one product that was better than the other? ??? was especially interested to hear about Ecomount from Biocare. Thanks, Rashmil _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 51 **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
