Correct me if I am wrong but CD11c is predominantly on dendritic cells and F4/80 is for predominantly macrophage/myeloid populations (therefore a good CD11b substitute). Are you sure you should use F4/80 as a CD11c substitute?
-----Original Message----- From: Patsy Ruegg <[email protected]> Date: Sat, 30 May 2009 10:44:14 To: 'gayle callis'<[email protected]>; 'Histonet'<[email protected]> Subject: RE: [Histonet] RE: CD11c for murine tissu An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use from Serotec, it works in frozen and ffpe tissue. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of gayle callis Sent: Saturday, May 30, 2009 8:45 AM To: 'Histonet' Subject: [Histonet] RE: CD11c for murine tissu You wrote: Hi all - I'm looking for a good antibody to use on mouse tissue (frozen) to look for CD11c - does anyone have a suggestion for a good antibody source? Thanks in advance - Melissa We us CD11c, HL3 clone, Armenian Hamster anti Mouse. Negative control is Armenian Hamster IgG1 from BD Pharmingen/Invitrogen. Tissues are fresh frozen sections fixed with our favorite acetone/absolute ethanol fixative, although you can use 4C acetone for 10 min on overnight air dried sections. I will be happy to send the other fixative recipe if you want it. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
