I've never read about fading caused by xylene. What about all people, who uses the film-coverslipper? That instruments demands on xylene as clearant. So if it would be true, there would be many faded slides in the archives. Perhaps your colleagues thought, that dehydration wasn't sufficient and the remaining water could cause fading. But after three 100% ethanol, I think, this fear has no cause. We use only one 96 and two 100 for the frozens and have never had problems.
Gudrun -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Lynn Dike Gesendet: Samstag, 20. Juni 2009 03:53 An: [email protected] Betreff: FW: [Histonet] xylene and frozen sections Lynn From: [email protected] To: [email protected] Subject: RE: [Histonet] xylene and frozen sections Date: Thu, 18 Jun 2009 19:53:52 -0500 Is there any literature that says xylene will fade progressive staining H&E (using Surgipath Harris Hematoxylin) over a period of years? Our frozen staining series after the eosin has two 95% ROH's and six 100% and then clear in Propar. I mistakenly replaced the last three 100% ROH with xylene when I rotated the series. I'm told that anyone who knows anything about chemistry knows this is a big deal and the sections will fade. Every lab I've worked in used xylene to clear frozen sections. I understand that I messed up because this isn't our proceedure, but I don't believe I sacrificed patient care. Am I wrong? Lynn > From: [email protected] > To: [email protected] > Date: Thu, 18 Jun 2009 19:47:14 -0500 > Subject: [Histonet] xylene and frozen sections > > > > > > Lynn > > > _________________________________________________________________ > Insert movie times and more without leaving Hotmail®. > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009 Insert movie times and more without leaving Hotmail®. See how. _________________________________________________________________ Hotmail® has ever-growing storage! Dont worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutoria l_Storage_062009_______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
