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Sent: Wednesday, July 29, 2009 1:01 PM
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Subject: Histonet Digest, Vol 68, Issue 38
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Contents of Histonet digest..."
Today's Topics:
1. Artifact in Muscle bx's frozen for extended time (Sharon Allen)
2. flash frozen tissue (Kalleberg, Kristopher)
3. Mouse Eyes (Jo-Ann Bader, Ms.)
4. SLIDE PRINTER (Janice Mitchell)
5. Re: Mouse Eyes (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Wed, 29 Jul 2009 09:27:24 -0500
From: "Sharon Allen" <[email protected]>
Subject: [Histonet] Artifact in Muscle bx's frozen for extended time
To: <[email protected]>
Message-ID:
<bb6adcd4b7abb045a09a7634ac15cc610bec8...@hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
I would like suggestions on how to eliminate or decrease the effects of
extended storage of muscle bx's in a -70°C freezer. We do approx. 150 muscle
bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's
indefinitely, (we have them all from the 80's on). We are asked fairly
regularly to go back to these cases for diagnostic & research purposes & find
many of them are compromised, readable but not optimum, I am assuming from
drying out from months/years of freezing.
We freeze them using the isopentane/liquid nitrogen method on a pc of cork with
OCT anchoring them. We store them in a small plastic tube with a screw top.
I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping
the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact?
Suggestions for long term storage would be very much appreciated.
Thanks
Sharon Allen
Senior Neuropathology Technologist
HSC
WPG, MB, CA
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Message: 2
Date: Wed, 29 Jul 2009 10:31:39 -0400
From: "Kalleberg, Kristopher" <[email protected]>
Subject: [Histonet] flash frozen tissue
To: <[email protected]>
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Hello All,
I will be running a study upcoming and we have decided not to run FFPE as we
normally do and have decided to use flash frozen. My big concern is what is
the proper way to fix all of these SKIN samples to use for IHC. Any
recommendations as to if prefix the samples in sucrose and then flash freeze in
OCT is best or any recommendations as to if post fixing (in acetone??) the
already sectioned, flash frozen sample in OCT is best. Any and and all help
will be greatly appreciated. Thank you.
Kris Kalleberg
------------------------------
Message: 3
Date: Wed, 29 Jul 2009 11:38:05 -0400
From: "Jo-Ann Bader, Ms." <[email protected]>
Subject: [Histonet] Mouse Eyes
To: "[email protected]"
<[email protected]>
Message-ID:
<76d119ef12c904418800ed67ccb2062905e2ab9...@exmbxvs1.campus.mcgill.ca>
Content-Type: text/plain; charset="us-ascii"
We have a new project pending with mouse eyes. The lens must remain intact.
What is the best method to process, embed and cut them? Paraffin or glycol
methacrylate?
Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W.
Room 312
Montreal, QC, Canada
H3G 1Y6
------------------------------
Message: 4
Date: Wed, 29 Jul 2009 11:42:27 -0400
From: "Janice Mitchell" <[email protected]>
Subject: [Histonet] SLIDE PRINTER
To: <[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=US-ASCII
Anyone out there in Histoland have any experience with Thermo slide mate? We
are looking into purchasing a few. Thanks for any input, Janice
------------------------------
Message: 5
Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT)
From: Rene J Buesa <[email protected]>
Subject: Re: [Histonet] Mouse Eyes
To: "[email protected]"
<[email protected]>, " Ms.Jo-Ann Bader"
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=iso-8859-1
I used to cut entire eyes of fish and lizards embedded in paraffin with great
results. The only thing is that on those (many years ago) days I used clearing
with Canada balsam followed by a short wash in benzene (not very safe) before
the paraffin infiltration.
René J.
--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <[email protected]> wrote:
From: Jo-Ann Bader, Ms. <[email protected]>
Subject: [Histonet] Mouse Eyes
To: "[email protected]" <[email protected]>
Date: Wednesday, July 29, 2009, 11:38 AM
We have a new project pending with mouse eyes. The lens must remain intact.
What is the best method to process, embed and cut them? Paraffin or glycol
methacrylate?
Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W.
Room 312
Montreal, QC, Canada
H3G 1Y6
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