Actually we have been using BD CD31 on mouse tissue with routine FFPE fixation with good results for a while now - Zn fixation not needed. Dako proteinase K 8 min, primary at 1:150, biotinylated secondary then Vector ABC with DAB substrate works well for us.
Cheers, Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: [email protected] ------------------------------ Message: 4 Date: Mon, 10 Aug 2009 15:14:05 -0400 From: "Amy Porter" <[email protected]> Subject: Re: [Histonet] CD31 working protocol To: "Zerfas, Patricia \(NIH/OD/ORS\) [E]" <[email protected]>, <[email protected]> Message-ID: <98811fbe01c548af99a3c982238f8...@histolab> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. It is well worth the results!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: [email protected] Web: www.humanpathology.msu.edu<http://www.humanpathology.msu.edu> ----- Original Message ----- From: "Zerfas, Patricia (NIH/OD/ORS) [E]" <[email protected]> To: <[email protected]> Sent: Monday, August 10, 2009 2:50 PM Subject: [Histonet] CD31 working protocol Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
