I am having some trouble getting good staining with the CD33 antibody from Novocastra. I am trying to stain FFPE bone marrow biopsies decalcified in Formical for 1 hour prior to processing. The staining is very weak even at a 1:25 dilution using a pH 9.0 antigen retrieval solution (recommended) in a pressure cooker. Has anyone had any luck with this antibody on bone marrow? I have tried this protocol with the blood clots sent with the bone marrow biopsies and it works just fine so I am guessing that the decal is affecting the staining. Any thoughts??
Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: [email protected] <mailto:[email protected]> _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
