Hi Histonetters we are having INTERMITTENT problems with brain frozens all conditions are the same ie. same tech, same cryostat, same freezing technique - sometimes there is freezing artefact in the sections and sometimes the sections look like they were cut from an FFPE block!!
unfortunately not all our pathologists are confident to make an IOC diagnosis on a smears so we are forced to resort to freezing. my tech is very experieince and says she can almost predict when looking at the tissue, that there will be artefact on the sections. pathologists are insisting on the FZ but complaining bitterly at the freezing artefact. I suspect the surgeon may be 'flooding' the tissue with saline but unless we breathe down their necks we cannot possibly know what goes on in the OR! All other frozens are fine - its just the very small neuro ones we are having a problem with and it is driving us to distraction - well, actually the Pathologists are doing that!! we are using cryospray, gently sprayed onto the tissue, which is covered with a small drop of fresh OCT, on top of an already frozen button of OCT which is kept in the cryostat there is no problem with other frozen sections, on other tissues, prepared in exactly the same way any and all observations/recommendations/suggestions will be greatfully accepted TIA -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
