corrected a spelling error!
Hi All
We are looking at rat issue which has had a gel subcutaneously
injected into it. We have routinely processed it and embedded it
into wax. The gel appears to survive processing and embedding, but
then seems to be pulled out or distorted during sectioning. We
would like to visualize it in tact to look at cells within the gel.
I have alternately embedded it in JB 4 resin (which is water
soluble and does not require dehydration. This method is so far the
best, however there are still some challenges.
i) There are sometimes bubbles in the resin that occur during
polymerization.
ii) Some gels, depending on formulations don't take up the resin
entirely, and therefore are still too soft to section.
We use a glass knife to section this resin. Staining is good, and
immunofluorescence in the form of ED-1 and Actin are successful.
Does anybody have any experience with this type of research related
problem?
Many Thanks
Melanie Black
Melanie Black
082 469 3352
Cardiovascular Research Unit
3rd Floor; Chris Barnard Building
Medical School;
Observatory. 7925.
University of Cape Town.
South Africa.
Melanie Black
082 469 3352
Cardiovascular Research Unit
3rd Floor; Chris Barnard Building
Medical School;
Observatory. 7925.
University of Cape Town.
South Africa.
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