thanks for this method we have been battling to get C4d FITC to work hopefully i can get a vendor here in the desert to source the reagents (that is a huge problem here) AbuDhabiAnnie
2009/10/16 James L Burchette <burch...@mc.duke.edu> > Frozen sections cut at 3-4 microns. Air dry 30 minutes. > Acetone fix for 5 minutes (room temp acetone), air dry slides > PBS wash x2 > C4d (diluted 1:500 in 1% BSA/PBS) incubated 1 hour. Mouse monoclonal C4d > is from AbDirect/Serotec > PBS rinse, wash x2 > Cy2 labeled goat anti-mouse IgG (diluted 1:100 in 1% BSA/PBS), incubated > 30 minutes (Cy2 GAR from Jackson ImmunoResearch Labs) > PBS rinse, wash x2 > Coverslip with fluorescence mounting media > Happy viewing > > I run this protocol everyday! > > Jim Burchette, HT(ASCP) QIHC > "A simple histotech from a little country hospital in North Carolina" > > > > "Foshey, Annette" <afos...@chw.org> > Sent by: histonet-boun...@lists.utsouthwestern.edu > 10/15/2009 04:56 PM > > To > " (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> > cc > > Subject > [Histonet] Procedure for manual C4D immunofluorescence staining > > > > > > > Histonetters, > Does anyone have a procedure they could share for manual C4D > immunofluorescence staining? > > Thanks in advance > > Annette Foshey, HT (ASCP) > Charge Tech in Histology > Children's Hospital of Wisconsin > 414-266-6580 Fax 414-266-2779 > afos...@chw.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet