Hi Patsy
I have tried RECA-1 and not had great success. The Fitzgerald one is
the best and we have researched this plenty.
One option for PFA fixed rat tissue is the Abcam VWF ( ab6994). I use
it 1:400 overnight incubation of primary with 10 minutes proteinase K
before hand, followed by Donkey anti-Rabbit Cy 3 (fluorescent stain).
Works well.
Melanie.
Melanie Black
082 469 3352
Cardiovascular Research Unit
3rd Floor; Chris Barnard Building
Medical School;
Observatory. 7925.
University of Cape Town.
South Africa.
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