Hi all! I was asked for advice, but have to give the question further to you.
We look for the best preparation of cornea for the atomic force microscope. A student wants to investigate the difference before and after the treatment of the cornea with a crosslinking drug. So any crosslinking fixative would bring artificial crosslinks and cannot be used. I think, that coagulating fixatives like ethanol, aceton etc. would also lead to artificial changes of the proteinstructure. The students wants to demonstrate the crosslinks of collagenfibers in cornea with the AFM. And the cornea should be seen as cross section to reach the interesting area. We will try to make frozen sections, but I doubt, that they will have the necessary quality and stability for AFM. So any tipp or hint is highly appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
