Dear Karen, We are using Invitrogen's monoclonal Anti-human IgG4 (Cat No. 05-3800) with a working dilution around 1/700. Although we are using BondMax stainer with 30 min, Epitope Retrieval solution 2 (alkaline) pretreatment from Leica, Ventana would give a good staining as well.
Young Sun Young Kwun Senior Hospital Scientist Immunohistochemistry Department of Anatomical Pathology Concord Hospital, Concord NSW Australia 61-2-97676075 (tel) 61-2-9767-8427 (fax) 0408868288 (mob) -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Bauer, Karen L. Sent: Tuesday, 10 November 2009 8:18 AM To: [email protected] Subject: [Histonet] IgG4 antibody Hello, I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP stainer. Is anyone out there using this antibody on their XT or Ultra? If so, could you please share with me the vendor you go through and what you are using for a protocol? Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
