Sorry I made a type "O" I meant to say Also, manufacturer's will
revalidate antibodies when they are close to their expiration date
and if that antibody has the same sensitivity. They will extend the
shelf-life date.
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: [email protected]
On Dec 7, 2009, at 5:02 PM, Akemi Allison wrote:
I agree with Tim. This is a very common practice for antibody
manufacturer's. Also, manufacturer's will revalidate antibodies
when they are close to their exploration date and if that antibody
has the same sensitivity. They will extend the shelf-life date.
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: [email protected]
On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote:
Kim,
Test a random sampling (10%) of the antibodies and see if they
work. If so, then they are most likely fine. Document the test.
Antibodies are very robust and the warmup probably won't damage
many, if any, of them.
BTW, When I worked for an antibody manufacturer I did a test once
as part of a stability problem investigation and left six
different antibodies in a 37C oven for up to 4 months. We tested a
sample of each weekly. All worked fine and some even worked better
even after 4 months!
The issue I would be concerned about is bacterial growth in any of
them. Keep an eye on that.
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-----Original Message-----
From: [email protected] [mailto:histonet-
[email protected]] On Behalf Of Kim Merriam
Sent: Monday, December 07, 2009 6:51 AM
To: Histonet
Subject: [Histonet] opinion please
Help!
My refrigerator died over the weekend. This fridge had most of my
antibodies and IHC reagents. When I came in, the temperature
inside the fridge was a balmy 37C!
Do you think any of my reagents are still usable?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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