My favourite for intense specific staining at the edges vs. faint specific staining in the center is poor fixation. I think drying artefacts or crush artefacts would lead rather to unspecific background staining.
Gudrun Lang Histolab, Akh Linz, Austria -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Karen Cai Gesendet: Montag, 11. Jänner 2010 20:01 An: [email protected] Betreff: [Histonet] Eliminating the edge effect in IHC/IF Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax <http://www.prosci-inc.com> www.prosci-inc.com _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
