Kim, how are you fixing the smear? Make sure to use acetone-free methanol. Are you using a manufactured stain kit? If so, which one? Karen Andover MA
>>> "Marshall, Kimberly" <[email protected]> 1/22/2010 4:26 PM >>> I am from a small Hospital where due to the size, Histology does the Bone Marrow's. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my Pathologist. I have added stain time, checked the pH of my stain, tried different rinse's and buffers, but still not getting it dark enough. I do go back to the stain for 45 mins or more and that will get it at least close to what they want. Is there anyone out there that can give me some advise on this stain? Thanks in advance for your time Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
