Hello Histonetters,
I am using Shandon M-1 embedding matrix for cryosectioning perfused, cryo-protected spinal cord. The embedding media allows me to section without the curling you frequently get with OCT compound. One thing I am noticing, however, is the M-1 media does not dissolve very well, even in several washes in water/PBS, and dehydrations in ethanol and xylene. Consequently, the M-1 residue stains along with the histological stain. OCT dissolves cleanly. Have any of you experienced this, or have any suggestions on how to avoid this problem? The M-1 media is a new bottle (will not expire until Dec, 2011). Thank you in advanced for your suggestions. Best regards, Joyce Kwan Elan Pharmaceuticals South San Francisco, CA ******************************************************** This communication and any files transmitted with it may contain information that is confidential, privileged and exempt from disclosure under applicable law. It is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient, you are hereby notified that any use, dissemination or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender. Thank you for your co-operation. ******************************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
