We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good Liz
________________________________ From: [email protected] on behalf of Sherwood, Margaret Sent: Wed 2/3/2010 3:10 PM To: [email protected] Subject: Re: [Histonet] TRAP assay - acid phosphatase Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) [email protected] The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
