Valerie, I would look at the fixation time of the tonsils and on their storage time. If the tonsils are fixed much longer than your lymphoma specimens a harsher pretreatment is necessary. If the control-tonsils are stored for long time the surface could suffer from oxidation. Do you have fresh tonsil for testing?
Gudrun -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Hannen, Valerie Gesendet: Mittwoch, 24. Februar 2010 17:48 An: [email protected] Betreff: [Histonet] Bcl-2 Hi everyone, I am hoping you all can help me solve a problem that I am having with my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but for the life of me can not get normal tonsil tissue to stain. I use a Retrieval solution with a pH of 8, use Avidin / Biotin solutions, incubate in the primary for 60 minutes. The tonsil tissues that I am testing are inhouse fixed and processed tissues. We use Zinc Formalin for our fixation. Now that I think of it, I am also having trouble getting a purchased control of Lymphoma tissue to stain. The kit that we are using is the Histostain + kit, and we do our Immuno's manually. What am I doing wrong or what am I not doing? Thanks in advance to any and all replies. Valerie Hannen, Histotechnologist Parrish Medical Center Titusville, Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
