I like this method too, and I would say it's one of the best. The only issue is high throughput. When freezing 100+ samples the floating tray becomes much more user friendly and believe it or not, the results are just as good.
--- On Wed, 2/24/10, Adam . <[email protected]> wrote: From: Adam . <[email protected]> Subject: Re: [Histonet] Snap Freezing Tissue To: "Andrea T. Hooper" <[email protected]> Cc: [email protected], "Laurie Colbert" <[email protected]>, "Steve Pike" <[email protected]> Date: Wednesday, February 24, 2010, 10:31 PM I learned to freeze tissue by taking a beaker and filling it with 2-methylbutane and then placing that beaker in a liquid nitrogen bath. You then take place your cryomold, hold it using some long forceps, and place it on the top of the 2-methylbutane. It will freeze the entire thing in about 10 seconds. Once it's frozen, you dip the entire mold into the 2-methylbutane. Seems to work well for me. Adam _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
