Refixing the slides is a usual procedure. RT over night or 2 hours 60°C oven.
The longer the acid step the brighter the red stain. Gudrun Lang -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von [email protected] Gesendet: Mittwoch, 07. April 2010 19:48 An: HistoNet Betreff: [Histonet] Refixing with Bouin's for Masson's Trichrome Hi Histonet, I am currently refixing my slides in Bouin's solution. Should I be refixing the entire tissue sample? What I mean is, should I deparrafinize my heart samples, refix in Bouin's, and reinfiltrate them before slicing with the microtome? Or is it more effective to just refix the slides with the heart sections on them? Also, my procedure for the Trichrome stain has me put the slides into phosphomolybdic acid for 5 minutes. I find that the slides come out of the procedure very dark and purple, instead of vibrant red as expected. Is the acid wash the problem? Should I reduce the time spent in the acid? Thanks! -Colin _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
