Eva, If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: [email protected] [[email protected]] On Behalf Of Sebree Linda A [[email protected]] Sent: Thursday, May 06, 2010 9:49 AM To: Eva Permaul; [email protected] Subject: RE: [Histonet] dehydration of hydrated slide....
Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: [email protected] Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
