I've read only the abstract and some infos regarding this article in the internet. This group used a short processing protocol (2:30), didn't they? I think that hampers the comparability with standard overnight processing. And I missed the longer fixation tests. They mentioned, that Goldstein left the tissue in 100% cold ethanol before processing. I assume, that ethanol-fixation is the cause for weaker staining in underfixed areas.
How are the commonly known edge-effects in ER-staining with stronger staining in the outer areas to explain, if not with insufficient fixation? It could be possible, that the longer incubation with processing reagents has effects on underfixed areas, that were not obvious in their experiment because of the short processing. Hm, I'm not convinced. Gudrun -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Richard Cartun Gesendet: Donnerstag, 06. Mai 2010 17:58 An: Histonet Betreff: [Histonet] Formalin fixation time for breast specimens There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
