Andrea - Wow this looks great. We do a lot of cardiomyocyte staining and
studying and this could be helpful. Thanks for sharing!
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
--On Wednesday, August 18, 2010 8:18 AM -0500 Andrea Marion
<[email protected]> wrote:
There is an immunofluorescence technique that uses wheat germ agglutinin
(WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the
cell periphery, leaving you with beautiful traces of the outside of each
cell. The cell area is then simple to analyze with ImageJ or any other
software program. It's often used to measure cardiomyocyte size in
hypertrophy studies. I can provide a protocol if requested.
If you have access to this article, there is a detailed description of the
technique and great images: Regional changes in myocyte structure in model
of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267:
H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C.
Greenfield Jr.
If not, here is a freely accessible publication. Figure 9, panel B shows
what the result looks like. Cardiomyocyte GATA4 functions as a
stress-responsive regulator of angiogenesis in the murine heart. J Clin
Invest. 2007; 117(11):3198.
http://www.jci.org/articles/view/32573/figure/9
Andrea Marion
Graduate Student
University of Illinois - Chicago
amario3 <at> uic <dot> edu
To: [email protected]
Subject: [Histonet] muscle stain
I am posting this for a friend. . They would like to stain muscle.
The eosin and hematoxylin stain gives us too much information since it
stains
the nucleus separately from the cytoplasm and confuses the image analysis
software. What we need is the measurement of the cell diameter only, a
simpler
stain. Thanks for any info you can give me. Deon Simon
Any help you can give her on how to measure or a stain will be very much
appreciated.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
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