Cornelia: After a "deadly" fixation, followed by a complete dehydration, clearing and paraffin wax infiltration, the only potentially surviving "entities" are prions. No cell will be viable after this treatment and if you get some positive results of viability with what ever test you use, I caution to believe those results. Your research director evidently is absolutely ignorant about tissue processing! René J.
--- On Wed, 8/18/10, Reuel Cornelia <[email protected]> wrote: From: Reuel Cornelia <[email protected]> Subject: [Histonet] Test for cell viability in paraffin section? To: [email protected] Date: Wednesday, August 18, 2010, 9:55 AM Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
