Have you validated this processing? Leaving the tissue in 70% alcohol for 48 hours is not standard, and thus requires all of the hassles associated with validation.
Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 [email protected] Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Phyllis Thaxton Sent: Wednesday, October 13, 2010 7:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; [email protected] Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" <[email protected]> To: "Mahoney,Janice A" <[email protected]>; Mike Pence <[email protected]>; Joyce Cline <[email protected]>; [email protected] Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; [email protected] Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:[email protected]] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; [email protected] Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; [email protected] Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: [email protected] Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 [email protected]<mailto:[email protected]> ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. 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