Richard, we had trouble with autofluorescence in brain tissue of the elderly. We have found that 1% Sudan Black B in 70% EtOH for 2 minutes before the secondary antibody works very well.
Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173 Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, October 18, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Re: Histonet Digest, Vol 83, Issue 20 (Weems, Joyce) 2. RE: AFB (Weems, Joyce) 3. RE: Re: Histonet Digest, Vol 83, Issue 20 (histot...@imagesbyhopper.com) 4. Re: AFB (Lee & Peggy Wenk) 5. autofluorescence (Edwards, Richard E.) 6. RE: IHC FILD (Stephanie Rivera) 7. (no subject) (Debora Probst) 8. Eosin (sgoe...@xbiotech.com) 9. Block release (Bernice Frederick) 10. RE: Block release (Helen Fedor) 11. RE: Block release (wanda.sm...@hcahealthcare.com) 12. Re: Eosin (Rene J Buesa) 13. clinical trial blocks (Tench, Bill) ---------------------------------------------------------------------- Message: 1 Date: Sun, 17 Oct 2010 18:46:54 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 To: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e16403a6810...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -----Original Message----- From: histot...@imagesbyhopper.com [mailto:histot...@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" <jwe...@sjha.org> wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpe...@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 2 Date: Sun, 17 Oct 2010 18:47:41 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] AFB To: Cheryl Crowder <ccrow...@vetmed.lsu.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e16403a6810...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I thought Fite's was more specific for leprosy.. whatever that bug is called now. j -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Saturday, October 16, 2010 10:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Sun, 17 Oct 2010 19:15:29 -0400 From: <histot...@imagesbyhopper.com> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 To: "'Weems, Joyce'" <jwe...@sjha.org> Cc: histonet@lists.utsouthwestern.edu Message-ID: <f75416dd10b6413e96ec8c85ac1a9...@hopperpc> Content-Type: text/plain; charset=US-ASCII Joyce, Certainly not *your* fault that *I* missed it! THANK YOU for reposting the link. :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Sunday, October 17, 2010 6:47 PM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -----Original Message----- From: histot...@imagesbyhopper.com [mailto:histot...@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" <jwe...@sjha.org> wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpe...@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). It may contain information that is privileged and > confidential. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, > please delete this message, and reply to the sender regarding the > error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/17/10 18:33:00 ------------------------------ Message: 4 Date: Sun, 17 Oct 2010 22:02:23 -0400 From: "Lee & Peggy Wenk" <lpw...@sbcglobal.net> Subject: Re: [Histonet] AFB To: "Cheryl Crowder" <ccrow...@vetmed.lsu.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <474b6e602a164b3c90484afe22963...@hp2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi - Can't find the original question - must have deleted it, so I'll answer through Cheryl's, if that's OK with her. If I remember, the question was something like - are there other microorganisms besides TB that stain with Kinyoun (or something like that). First, any mycobacterium (acid fast bacteria AFB) will stain with Ziehl-Neelsen, Kinyoun, Auramine-Rhodamine or Fites (hence anything staining with these stains is called AFB positive, meaning staining with these AF stains) . So that means Mycobacterium tuberculosis (TB), Mycobacterium avium intracellular (found in birds and I've seen it in spleen and bone marrow of immune suppressed patients), Mycobacterium paratuberculosis (found in intestine of infected cows and goats, causing something similar to Crone's disease), and a lot of other Mycobacterium are AFB positive staining with these procedures. Mycobacterium leprae (leprosy) is a very thin walled mycobacterium, so needs the Fites stain with the peanut oil (or any other oil, such as mineral oil) to coat the leprosy microorganism, so it can withstand decolorization with aqueous alcohol. But Fites can also be used to demonstrate any of the Mycobacterium. These AFB stains will also demonstrate the sulfur clubs of actinomyces, and the filamentous strands of nocardia are also weakly AFB positive. (Sort of bacteria with some fungus characteristics.) Cryptosporidium found in the intestine (causing severe diarrhea) can also stain AFB positive. And there are bacterium (mycobacterium or others) in tap water that are AFB positive, which can adhere to your slides from the flotation bath water, or from the tap water. Usually non-pathogenic AFB, but I don't know their names. And I'm sure there are other less common bacteria that I've forgotten or never known about that will stain AFB positive, which are not in the Mycobacterium genus. And let's remember that hair follicles, sperm (heads with lipids), lipofucsin (wear and tear pigment of lipids) also stain AFB positive. And also Russell bodies (inclusions of antibodies in plasma cells) will stain AFB positive. And keratin which is dense can simply require longer differentiation, or else it will retain the red color of the dye. So, yes, Amy, there are other things that stain positive with Kinyoun that are not TB/mycobacterium. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Cheryl Crowder" <ccrow...@vetmed.lsu.edu> Sent: Saturday, October 16, 2010 10:48 AM To: <histonet@lists.utsouthwestern.edu> Subject: [Histonet] AFB > Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast > bacteria. The Fite's stain is usually considered specific for > mycobacteria. > Cheryl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 18 Oct 2010 10:51:36 +0100 From: "Edwards, Richard E." <r...@leicester.ac.uk> Subject: [Histonet] autofluorescence To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <7722595275a4dd4fa225b92cdbf174a1e8db32c...@exc-mbx3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Any information on current methods to counter autoflourescence in formalin fixed paraffin processed tissues would be much appreciated. Many thanks Richard Edwards Leicester University U.K................ ------------------------------ Message: 6 Date: Mon, 18 Oct 2010 08:13:07 -0500 From: Stephanie Rivera <stephanie.d.riv...@gsk.com> Subject: [Histonet] RE: IHC FILD To: Debora Probst <debora.pro...@crhs.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <14a77a757e86bc499a34e86b47649b64054fc6b...@019d-namsg-05.019d.mgd.msft.net> Content-Type: text/plain; charset="us-ascii" My company does not recognize the certification. It was just for my personal development. Some hospitals do recognize it as a specialty but I don't know if you get an increase. Check with your manager. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, October 15, 2010 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC FILD Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 18 Oct 2010 10:51:08 -0400 From: "Debora Probst" <debora.pro...@crhs.net> Subject: [Histonet] (no subject) To: <histonet@lists.utsouthwestern.edu> Message-ID: <4843cffc8dc9a54e88e4ab3f5a50689819c1a...@crhs_exch.nterprise.crhs.net> Content-Type: text/plain; charset="us-ascii" I would just like to thank everyone for their response back on IHC certification. What a great resource for our field (Histology). Again thank you everyone. Debora Probst Columbus Regional, Columbus Ga. ------------------------------ Message: 8 Date: Mon, 18 Oct 2010 07:53:00 -0700 From: <sgoe...@xbiotech.com> Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Message-ID: <20101018075300.9e2d9aa830e8449a2412eb1e4f2f067e.1df96a717f....@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" Once upon a time I heard that eosin fluoresces is this true? color does it show up? Could this be used as a sort of back gr stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician 8201 East Riversid Austin, Texas (512)386-2907 ------------------------------ Message: 9 Date: Mon, 18 Oct 2010 10:04:23 -0500 From: "Bernice Frederick" <b-freder...@northwestern.edu> Subject: [Histonet] Block release To: <histonet@lists.utsouthwestern.edu> Message-ID: <0ad641bb647144bcbc1ae4cd68c18...@lurie.northwestern.edu> Content-Type: text/plain; charset="US-ASCII" Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ------------------------------ Message: 10 Date: Mon, 18 Oct 2010 11:12:08 -0400 From: Helen Fedor <hfe...@jhmi.edu> Subject: RE: [Histonet] Block release To: Bernice Frederick <b-freder...@northwestern.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3201cf51728f6048a24fa3afffeef1d31781f6f...@jhemtexvs3.win.ad.jhu.edu> Content-Type: text/plain; charset="us-ascii" Hello, Our institution does release blocks, the requirement is that a patient consent is signed and we have an MTA (Material Transfer Agreement) For the study and appropriate IRB's in place for the study. It is a lot to set up, but after this is done for one study any following studies are easier to handle. Helen -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 18 Oct 2010 10:22:33 -0500 From: <wanda.sm...@hcahealthcare.com> Subject: RE: [Histonet] Block release To: <b-freder...@northwestern.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <9e2d36ce2d7cba4a94d9b22e8328a3ba1395e57...@nadcwpmsgcms03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Good Morning to All, We, as often as possible, try not to release blocks for studies or legal cases. Most studies will let you submit 10-20 unstained recut slides on Plus slides. On legal cases, we always give recuts if there is enough tissue. When slides will not suffice, we will send the block and keep a record of the block being sent. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 18 Oct 2010 08:51:43 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu, sgoe...@xbiotech.com Message-ID: <9500.31952...@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if you observe a solution with transmitted light. Ren? J. --- On Mon, 10/18/10, sgoe...@xbiotech.com <sgoe...@xbiotech.com> wrote: From: sgoe...@xbiotech.com <sgoe...@xbiotech.com> Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Date: Monday, October 18, 2010, 10:53 AM ???Once? upon a time I heard that eosin fluoresces is this true? = ; What ???color? does it show up?? Could this be used as a sort of back gr= ound ???stain so you can tell exactly how many cells are in the field? ???Sarah Goebel, B.A., HT (ASCP) ???Histotechnician ???= = XBiotech USA Inc. ???8201 East Riversid= e Dr. Bldg 4 Suite 100 ???Austin, Texas=???78744 ???(512)386-2907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 18 Oct 2010 08:58:44 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] clinical trial blocks To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii The fundamental philosophy that we have is that we do not want to stand in the way of the patient receiving any kind of treatment. That being said, we also have the policy that whether or not we "own" the material (patients are actually suppose to sign an admission consent that says they give up any ownership rights), we are clearly the legal custodians of the material, and as such are responsible for insuring that no irreparable distruction occurs. So, we prefer to not send out blocks unless the clilnical trial says there are no alternatives. We contact them and ask if we can send recuts from which they can obtain the information they desire. We also review what we have. If we have only one section, we cut a recut for the file before sending out any blocks. If we have only one block, with not much in it, we again discuss the issue with the trial coordinator. We do insist on return of the block, and assuming that there is an outside review of the material, we insist on a copy of the report. We also have the patient sign a release informing them that they need to understand that this process may exhaust any tissue that would be useful for some other new treatment which may come up down the line. Since we have to pull this material for review and usually make recuts, we bill the clinical trial. Years back, they never had any budget allowances for this, and it was always a hassle. More recently, we get a lot less BS. They know they have to pay, and they do. Unfortunately, nothing is free any more. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. 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