It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. Not sure on the dilution, but maybe someone else knows.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [valerie.han...@parrishmed.com] Sent: Monday, October 25, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique ________________________________ From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet