We incubate the trichrome-slides after deparaffination for 2 hours in 56°C, then rinse in warm tapwater until the slides are colourless. Then another rinse in distilled water, because I don't want carry over from tapwater into the weigert's iron hematoxylin. ...
If you let bouins in the tissue, it acts like a stain. Think of vanGieson staining solution (also called picrofuchsin). It sounds like the processing after bouin-fixation and the trichrom staining are mixed up. Or the protocols of trichrom-staining of NBF-fixed and bouin-fixed tissue are mixed up. Gudrun -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von wassan alkadhumi Gesendet: Dienstag, 28. Dezember 2010 15:50 An: [email protected] Betreff: [Histonet] bouin solution Dear Histonet I have question concerning bouin solution, we use it in masson trichrome before starting the stain. we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled. Then we wash the slides using running water first and then distilled water, but according to what i red, when using bouin solution,the tissue should be washed with (50-70)% alcohol to prevent deterioration of staining in time. now i should mention that in the our masson trichrome procedure there is no washing with alcohol but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
