Dear all,
I had two antibodies made (in rabbit) for the same peptide. It was the first time I did this : the firm designed the immunization peptide, injected and bleed the rabbits and purified the antibody. I have tested it on paraffin sections: all background, no staining where it should be. I tried different retriever buffers and detection with dab and fluorescence. I have tested it on western blot: a lot of background, dirty lanes. To get a clearer view I performed a co-IP. Still not really great. Can anybody help me on this issue? I just want it to work for staining. Thanks a lot in advance! Barbara Verstraeten, Drs. Evolutionary Developmental Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 B-9000 Ghent Belgium tel: ++32/(0)9 264 52 31 fax: ++32/(0)9 264 53 44 http://www.evodevo.ugent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
