Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ----- Original Message ----- From: "Scott, Allison D" <allison_sc...@hchd.tmc.edu> Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] H&E Stain To: histonet@lists.utsouthwestern.edu
> Hello to all in histoland and Happy New Year. We are > having issues with > our H&E stain. The nuclei are staining very blue to purple > and the > mucin is staining blue to purple-blue. It is difficult to > see the > nuclear detail. The mucin is obscuring things. We > have not changed our > process for staining or processing. The funny thing is > that it is only > in the Biopsy cases, and it is every few slides. The > surgical cases > are all right. We checked the alcohol and xylene for > water, and there > is not any. My tech changed out the stain and we are > staining a new > batch of slides. If anyone has any idea what is wrong, any > help would > be greatly appreciated. I have gone over our processes and > nothing has > changed. The reagents are the same, the staining times are > the same, > and the processing times are the same. We are using the > Shandon Gemini > stainer and VIP processor. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately > notify the > sender by return e-mail and delete this e-mail and any > attachments from > your computer system. > > To the extent the information in this e-mail and any attachments > contain > protected health information as defined by the Health Insurance > Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or > privileged. This e-mail may also be confidential and/or > privileged under > Texas law. The e-mail is for the use of only the > individual or entity named > above. If you are not the intended recipient, or any > authorized > representative of the intended recipient, you are hereby > notified that any > review, dissemination or copying of this e-mail and its > attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet