How can I eliminate the background or as you say, non-specific staining?
I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
