For the most part you cannot section fat on a cryostat because of the oilyness (is that a word). You can fix it and section it? Good Luck
Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Wednesday, March 02, 2011 4:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fat tissue Has anyone experience of cryostat sectioning of fat tissue? This would be rodent fat pads, particularly the gonadal fat pad which usually consists largely of adipocytes. What temperature would you cut at? Is there anything that could be used to infiltrate the tissue prior to sectioning to provide more support? Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet