We let our smears air dry and then we stain them on the Wright stainer, in which the first step is a fixative. We reserve one slide for an FE stain, this slide is fixed in Methanol for 10 mins, then continue per usual with the FE stain. Hope this helps!
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l [email protected] Debra Siena <[email protected]> Sent by: [email protected] 03/02/2011 10:49 AM To "[email protected]" <[email protected]> cc Subject [Histonet] bone marrow aspirations Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation. I am not embarrassed to say that it has been awhile since I worked with them. With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap fixative) or something else? I do thank you for your help. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 [email protected]<mailto:[email protected]> | www.statlab.com< http://www.statlab.com/> _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
