Diana, I have a lot of experience with decal reagents and Formic acid is the best in my experience for IHC. It is not as fast as the rapid decals but is the kindest at not adversely affecting the antigens and more gentle on iron leaching, although all decals will leach iron, we usually do iron on the smear or clot section. Not only does the formic acid leach the iron so does the alcohols and solvents in tissue processing.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Diana Martinez-Longoria Sent: Thursday, March 24, 2011 11:01 AM To: [email protected] Subject: [Histonet] bone marrow biopsy decals Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance! Diana Martinez-Longoria Histotechnician HT(ASCP)CM El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax [email protected] Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
